Metaara - Ustar CPA Test Kits

 

Metaara Medical Technologies Inc. supplies Metaara - Ustar CPA test kits for research use only.

Cross Priming Amplification, CPA

  • One temperature: amplification without PCR machine
  • One enzyme: good for reagent stability and assay optimization
  • Flexible: primer and probe designs tailoring your purpose
  • Amplification time: one hour or less
  • Glassified reagents: stable for ambient temperature transport and storage

The test kits are fast, easy to use and take only 40 minutes from sampling to detection. All this in three simple steps:

Step 1: Sample Preparation & Nucleic Acid Application :: Use Ustar rapid nucleic acid extraction kit which employs an easy specimen liquefying(for sputum), centrifuging and boiling method; Or use Ustar nucleic acid extraction device(Fig.1). This procedure takes only 20 minutes, and is instrument-free. You can also choose any other nucleic acid extraction methods commonly-used in laboratories.

Figure 1

Figure 1: Instrument-free Nucleic Acid Extraction Device

Step 2: Isothermal Amplification :: Transfer the prepared amplification template into a 0.2ml tube for CPA amplification, which can be accomplished with any incubator that keeps a constant temperature, such as a water bath, a dry heater, or a PCR machine. CPA amplification takes 30 to 60 minutes at 63°C.

Step 3: Rapid detection & Read-out :: Place the CPA reaction tube into the cartridge; Insert the cartridge into the disposable device and lock (Fig.2). Read result in 15 minutes (Fig.3). The disposable device, embedded with lateral-flow DNA amplicon detection strip, is completely sealed when locked, preventing cross contamination effectively.

Figure 2

Figure 2: Rapid Detection with Disposable Device

Figure 3

Figure 3: Amplicon Detection Strip and Read-out

Download sample instructional pamphlet.